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Guide to MELISA® Lyme testing
Please read the instructions and complete the Requisition form prior to collecting blood.
6. Payment & Results
You will be invoiced 2 weeks after your sample arrives at the lab. Payment can be made through bank transfer, or
online through PayPal via debit or credit card. Results cannot be released until payment is received.
MELISA Diagnostics Ltd • 636-552-9637 • info@melisa.org • melisa.org page 1 of 2
2. Medication & health
MELISA testing measures an immune response. Any drug that suppresses the immune system may affect test
results. Certain medications, such as steroids, must be avoided for 3 weeks to ensure accurate results. In addition,
antibiotics must be avoided for 4 weeks. For a list of medication and factors that may affect testing, see
melisa.org/medication. If testing shows that your cells are not responding adequately due to medication taken, $130
will be charged.
1. Diagnostic use
A positive reaction in the MELISA Lyme test demonstrates current active infection with Borrelia burgdorferi. The test
improves laboratory diagnosis of Lyme Borreliosis by confirming an active infection in patients with clinical suspicion
of Lyme disease.
The MELISA test covers the following antigens: Borrelia afzelii, Borrelia garinii and Borrelia burgdorferi senso stricto
p41-antigen internal fragments, peptide mix OspA/VlsE of Borrelia garinii, afzelii, senso stricto and full antigen lysate of
Borrelia burgdorferi sensu stricto.
3. Blood draws
Blue top citrate tubes • Use 5 tubes
Red top serum tube • One 9 ml red top serum tube is required for autologous serum. If possible, spin the
tube for 10 minutes at 1800 - 2200 rcf/g and keep at room temperature.
4. Shipping
Blood should be sent via FedEx on Mondays and Tuesdays only, arriving no later than 48 hours after blood is
collected. Check for information about public holidays in Germany before shipping: melisa.org/calendar
Ship the blood sample and the completed Request form to:
Invitalab, MELISA Department
Stresemannallee 4b
414 60 Neuss
Germany
Phone +49 2131 125 9690
7. Queries
If you have any questions about the testing procedure or about your test results, please contact us on
info@melisa.org or 636-552-9637.
5. Cost of testing
MELISA Lyme testing $380
Winter kit fee $65
Shipment by FedEx approx $200
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Requisition form: MELISA® Lyme testing
Complete and include with blood sample. See previous page for Guide to testing.
Patient
Name Date of birth
Address
Email Tel
Sample collectionDate of blood draw Collection time
Please tick to confirm that you understand:
1) Immuno suppressants, such as steroids, as well as antibiotics, must be
avoided for 3 weeks to ensure accurate results. Systemic infection may also
affect the results.
2) After blood is collected, it must be delivered to the lab within 48 hours,
or cells will not be viable.
Invitalab laboratory label here
(do not fill)
Lyme testing
MELISALyme testing $380
Testing for Borrelia afzelii, Borrelia garinii, Borrelia burgdorferi sensu stricto
Zip code
Clinic
Prescribed by
Address
Email Tel
Zip code
Patient history
MELISA Diagnostics Ltd • 636-552-9637 • info@melisa.org • melisa.org page 2 of 2
Clinic
MM / DD / YYYY HH : MM
Please fill in as much data as possible as this will help with evaluating the test results.
Yes No Date
IgM IgG
IgM
pos.
IgG
neg.
Have you had a tick bite?
Do you have a red rash (Erythema migrans)?
Do you have neurological symptoms (Neuroborreliosis)?
Do you have chronic arthritis?
Do you have other symptoms?
Have you received any antibiotic therapy
(medication, length of treatment, etc)?
Have you had any of these serological tests? (serum/liquor)
EIA:
BLOT:
Oligocional bands:
12.04.2023