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Status: Currently O

cial on 16-Feb-2025

O

cial Date: O

cial as of 01-May-2022

Document Type: USP Monographs

DocId: GUID-A7836D18-5329-4E21-ADE5-4E684D475BDC_4_en-US

DOI: https://doi.org/10.31003/USPNF_M65840_04_01

DOI Ref: qos8u

© 2025 USPC

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Plantago Seed

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DEFINITION

▲Plantago Seed consists of the cleaned, dried, ripe seed of Plantago afra L. (formerly P. cynops L.) known in commerce as black psyllium; of

Plantago arenaria Waldst. & Kit. (formerly Plantago indica L. or P. psyllium L.) known in commerce as Spanish, French, or another source of

black psyllium; or of Plantago ovata Forssk. (formerly Plantago ispaghula Roxb.), known in commerce as ispaghula seed, blond psyllium, or

Indian plantago seed (Family Plantaginaceae).▲ (USP 1-May-2022)

CONTAMINANTS

• ARTICLES OF BOTANICAL ORIGIN 〈561〉, Pesticide Residue Analysis: Meets the requirements

SPECIFIC TESTS

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• Botanical Characteristics

Macroscopic

▲Plantago afra whole seed: Ovate to narrowly ovate-elongated to oblong, concavo-convex; mostly from 1.3–2.7 mm in length, rarely up to 3

mm, and from 600 μm to 1.1 mm in width, "boat-shaped". It is light brown to moderate brown, darker along the margin, and glossy; the

convex dorsal surface exhibits a lighter-colored longitudinal area extending nearly the length of the seed and represents the embryo lying

beneath the seed coat, and shows a sometimes indistinct transverse groove nearer the broader end. The concave ventral surface has a

deep cavity, in the center of the base of which is an oval, yellowish-white circular hilum.

Plantago arenaria whole seed: Ovate-oblong to elliptical, concavo-convex, "boat-shaped"; measures 1.6–3 mm in length and 1–1.5 mm in

width. Externally it is dark reddish brown to moderate yellowish brown, irregular, glossy to dull, rough, and reticulate; the convex dorsal

surface has a longitudinal lighter-colored area extending lengthwise along the center and beneath the seed coat, and a median transverse

groove, dent, or

ssure. The ventral surface has a deep concavity, the edges somewhat

attened and frequently forming a sharp indented

angle with the base of the cavity, the latter showing a light-colored oval hilum.

Plantago ovata whole seed: Broadly elliptical to ovate, "boat-shaped", from 2–3.5 mm in length and from 1–1.5 mm in width. It is pale brown

to moderate brown with a dull surface; the convex surface has a small, elongated, glossy brown spot. The concave surface forms a deep

cavity, in the center of the base of which is a hilum covered with a thin membrane.▲ (USP 1-May-2022)

Odor and taste: All varieties of Plantago seed are nearly odorless, ▲taste weak, and are mucilaginous.▲ (USP 1-May-2022)

Microscopic: Plantago seed is reniform in median transverse sections. Its seed coat has a colorless epidermis of mucilaginous cells whose

radial and outer walls break down to form layers of mucilage when brought into contact with water, and a reddish-brown to yellow pigment

layer in the seeds of ▲P. arenaria,▲ (USP 1-May-2022) a broad endosperm with thick-walled outer palisade cells, and irregular inner endosperm

cells; and a straight embryo extending lengthwise through the center. The endosperm and embryo cells contain

xed oil and aleurone

grains, the latter are round, oval, pyriform, or irregularly shaped, measuring 2–8 μm in diameter.

Change to read:

• Water ABsorption

Sample: 1 g of Plantago Seed

Analysis: Place the Sample in a 25-mL graduated cylinder, add water to the 20-mL mark, and shake the cylinder at intervals during 24 h. Allow

the seeds to settle for 12 h, and note the total volume occupied by the swollen seeds.

Acceptance criteria: ▲

▲ (USP 1-May-2022) NLT 8 mL

• ARTICLES OF BOTANICAL ORIGIN 〈561〉, Methods of Analysis, Foreign Organic Matter: NMT 0.50%

• ARTICLES OF BOTANICAL ORIGIN 〈561〉, Methods of Analysis, Total Ash: NMT 4.0%

• ARTICLES OF BOTANICAL ORIGIN 〈561〉, Methods of Analysis, Acid-Insoluble Ash: NMT 1.0%

OFFICIAL

2/16/25, 11:58 AM USP-NF Plantago Seed

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ADDITIONAL REQUIREMENTS

• PacKaGinG and StoraGe: Preserve in well-closed containers, and secure against insect attack.

Auxiliary Information - Please check for your question in the FAQs before contacting USP.

Topic/Question Contact Expert Committee

PLANTAGO SEED

Nam-Cheol Kim

Scienti

c Liaison

BDSHM2020 Botanical Dietary Supplements

and Herbal Medicines

REFERENCE STANDARD SUPPORT

RS Technical Services

RSTECH@usp.org

BDSHM2020 Botanical Dietary Supplements

and Herbal Medicines

Chromatographic Database Information: Chromatographic Database

Most Recently Appeared In:

Pharmacopeial Forum: Volume No. 46(6)

Current DocID: GUID-A7836D18-5329-4E21-ADE5-4E684D475BDC_4_en-US

DOI: https://doi.org/10.31003/USPNF_M65840_04_01

DOI ref: qos8u

OFFICIAL

2/16/25, 11:58 AM USP-NF Plantago Seed

www.webofpharma.com